Douglas J. Taatjes, PhD

Education:

Graduate School
Kansas State University, Manhattan, KS
Biocenter, University of Basel, Basel, Switzerland

Training:

Residency

Fellowship

Specialty:

Certifications:

Academic Appointments:

Biography:

Dr. Taatjes has been Director of the Microscopy Imaging Center in the College of Medicine at the University of Vermont since 1994. He has received the Robert Feulgen Prize of the International Association of Histochemists, serves on many grant review committees, and on the editorial board of Histochemistry and Cell Biology, and The Journal of Histochemistry and Cytochemistry, and is currently editing the book Cell Imaging Techniques.

Major Research Interests:

Dr. Taatjes utilizes sophisticated microscopy-based imaging techniques to characterize cardiovascular pathophysiology.  He is elucidating the role of plasminogen activator inhibitor type-1 in atherosclerotic lesion formation. Computer-assisted image analysis is being applied to captured confocal images for determination of the cellular and biochemical content of lesions.  Confocal scanning laser microscopy is used to clarify effects of anti-platelet therapy on the reactivity of human platelets.  Atomic force microscopy is delineating events in blood clot formation and molecular mechanisms responsible for the anti-phospholipid syndrome.

Major Current Grant Support

NIH (R01 HL61331) "Reduction of Annexin A5 in Antiphospholipid Pregnancy Loss"
04/01/07 - 03/31/12  Rand (PE)

NIH (R01 HL842000) "Reciprocol Regulation of SDF-1 between Marrow and Lung during Acute Lung Injury"
05/01/06 - 04/30/11 Surratt (PI)

Publications:

Representative Publications From a Total of 127

Taatjes, D.J., Wadsworth, M.P., Schneider, D.J., and Sobel, B.E. (2000) Improved quantitative characterization of atherosclerotic plaque composition with immunohistochemistry, confocal fluorescence microscopy, and computer-assisted image analysis. Histochem. Cell Biol. 113, 161-173.

Wadsworth, M.P., Sobel, B.E., Schneider, D.J., and Taatjes, D.J. (2002) Delineation of the evolution of compositional changes in atheroma. Histochem. Cell. Biol. 118, 59-68.

Rand, J.H., Wu, X-X, Quinn, A .S., Chen, P.P., McCrae, K.R., Bovill, E.G., and Taatjes, D.J. (2003) Human monoclonal antiphospholipid antibodies disrupt the annexin A5 anticoagulant crystal shield on phospholipid bilayers: Evidence from atomic force microscopy and functional assay.  Am. J. Pathol. 163, 1193-1200.

Wadsworth, M.P., Sobel, B.E., Schneider, D.J., Tra, W., van Hirtum, H., and Taatjes, D.J.  (2006)  Quantitative analysis of atherosclerotic lesion composition inmice.  Methods Mol. Biol. 319:137-152.

Bostrom, M.A., Boyanovsky, B.B., Jordan, C.T., Wadsworth, M.P., Taatjes, D.J., de Beer, F.C., and Webb, N.R.  (2007)  Group v secretory phospholipase A2 promotes atherosclerosis: evidence from genetically altered mice.  Arterioscler. Thromb. Vasc. Biol. 27, 600-606.

Clough, M.H., Schneider, D.J., Sobel, B.E., White, M.F., Wadsworth, M.P., and Taatjes, D.J.:  (2005)  Attenuation of accumulation of neointimal lipid by pioglitazone in mice genetically deficient in insulin receptor substrate-2 and apolipoprotein E.  J. Histochem. Cytochem. 53, 503-610.

Zaman, A.K.M.T., Fujii, S., Schneider, D.J., Taatjes, D.J., Lijnen,  H.R., and Sobel, B.E.:  (2007)  Deleterious effects of  lack of cardiac PAI-1 after coronary occlusion in mice and their pathophysiologic determinants.  Histochem. Cell Biol. 128, 135-145.

Taatjes, D.J., Wadsworth, M.P., Zaman, A.K.M.T., Schneider, D.J., and Sobel, B.E.:  (2007)  A novel dual staining method for identification of apoptotic cells reveals a modest apoptotic response in infarcted mouse myocardium.  Histochem. Cell Bio. 128, 275-283.

Taatjes, D.J., Sobel, B.E., and Budd, R.C.:  (2008)  Morphological and cytochemical determination of cell death by apoptosis.  Histochem. Cell Biol. 129, 33-43.

Taatjes, D.J., Wadsworth, M.P., Quinn, A.S., Rand, J.H., Bovill, E.G., and Sobel, B.E.:  (2008)  Imaging aspects of cardiovascular disease at the cell and molecular level.  Histochem. Cell Biol., in press.